Diseases of field, vegetable, orchard and plantation crops of India and their control; causes and classification of plant diseases; principles of plant disease control biological control of diseases; seed health testing, Integrated pest management-concepts and components; host plant resistancebiological control of insect pests; genetic manipulation of insects for their control; pesticides, their formulation, classification and safe use; behavioural methods; insect growth regulators; biotechnological approaches in IPM
Unit II Secondary metabolites:
Concept of secondary metabolites, their applications in agriculture and health industry.In vitro production of secondary metabolites: introduction to secondary metabolism, significance of cell differentiation, selection, downstream processing, influence of culture conditions on accumulation of secondary metabolites, immobilization of cells for enhanced production of secondary products, biotic and abiotic elicitation. Different techniques involved in isolation, purification and characterization of useful secondary metabolites from cultured cells
Unit III Transgenic techniques in plant biotechnology:
introduction of foreign gene into plants, basics of tumor formation, hairy root culture and its uses, features of Ti &Ri plasmid, mechanism of DNA transfer, role of virulence gene, use of reporter gene, multiple gene transfers, vector less or direct DNA transfer, particle bombardment, electroporation, microinjection, chloroplast transformation. Applications of plant transformation for enhancing resistance to pests, productivity & performance, nutritional value, modification of ornamental plants, bioengineered food, edible vaccines, plantibodies, biopharming.
Unit IV Functional Food: What is functional food? Functional food from plant sources, safety issues. Algal and Moss biotechnology: Biotechnological importance of algae, Growth in laboratory, algal farming, Techniques involved in algae biotechnology, Genetic engineering of algae for enhanced production of industrially important products, Biotechnology involving Cyanobacteria. Biofuels.Synthetic Biology: Introduction and applications in PTC
- Teacher: Sushama Patil