Animal cell culture

Unit - I: Laboratory design, aseptic techniques, types of culture and cryopreservation

(15 Hrs.)

1. Design of Tissue Culture Laboratory

2. Equipments : Laminar Flow Hoods, CO2 incubator, Microscopes, centrifuge,

Refrigerators and Freezers, pipetting aids, Miscellaneous Equipments.

3. Glass wares/plastic wares and filters for tissue culture

4. Basic Aseptic Techniques

5. Primary cell culture, Established cell line, transformed cell line

6. Cryopreservation for Storage and shipment

Unit - II: Growth media and Basic Techniques of mammalian cell culture (15 Hrs.)

1. Physical requirements and Nutritional Requirements of Cells

2. Natural media

3. Basal salt solution (BSS)-Various types

4. Minimum Essential Medium( MEM)

5. Serum dependent defined media

6. Serum independent defined media – Cell specific media

7. Antibiotics in media

8. Types of cell cultures – Open and closed cell cultures

9. Monolayer, Suspension, Clonal culture, Mass culture: micro carrier culture, Stem

cell cultures (ESC)

Unit - III: Biology and Characterization of cultured cells and applications of Animal cell

culture (15 Hrs.)

1. Viability measurement and cytotoxicity

2. Contamination Testing of Culture

3. Karyotyping

4. Measurement of growth parameters

5. Cell cycle analysis and Synchronization of cultures

Unit - IV: Applications of Animal cell culture, Cell surgery and tissue engineering

1. Evaluation of Chemical carcinogenicity, Cell malignancy Testing

2. Uses of Embryonic stem cells and Pluripotent stem cells

3. Hybridoma cell preparations and their properties

4. Surgical manipulation of in vitrofertilization : ICSI, Assisted zona hatching,

cytoplasmic transfer

5. Capillary culture Unit

6. Techniques for culturing differentiate