The goal of this course is to introduce biotechnology methods in plants. The objective of the course is to give students new knowledge and widening of the knowledge acquired in other course by handling of classical and modern plant biotechnology processes, including breeding of healthy plants, plants with improved characteristics and plants for biomolecule production. Understanding of biotechnological processes has also applicative value in pharmaceutical and food industry, in agriculture and in ecology.

BT-341: Plant Biotechnology (60)

(Prerequisite: B Sc Life Science)

UNIT-I (15)

Plant physiology and its significance in agriculture; physical properties and chemical constitution

of protoplasm, concept of diffusion and osmosis, plant cell water relation – imbibition,

absorption and translocation of water and nutrients, transpiration, guttation, Photosynthesismodern

concept and the factors affecting photosynthesis, nitrogen fixation

UNIT- II (15)

Introduction to plant biotechnology: history, concept of totipotency, heterogeneity,

cytodifferentiation, preparation of explant. Incubation systems: culture room, green house and

shade house, advantages and limitations of each system. Tissue culture media: general

introduction, composition, sterilization. Role of macro and micro nutrients in plant growth and

effects of their deficiency. Plant Hormones: types, role in plant development & mechanism of



Initiation, maintenance and importance of callus culture, suspension culture and single cell

culture, types and applications of single cell culture, organ culture, protoplast culture and its

applications, isolation and types of protoplasts fusion, seed dormancy and germination, somatic

embryogenesis its types and applications, encapsulation and production of synthetics seeds,

development of somatic hybrids to overcome the incompatibility barriers.

UNIT IV (15)

Anther culture and production of haploid plants and homozygous lines, embryo culture and

embryo rescue, pollen, ovary culture and their applications, germ plasm preservation.

Micropropagation: principle, vegetative regeneration by-shoot tip, meristem, axillary and

adventitious shoot initiation, organogenesis and production of pathogen free plants, hardening of

tissue cultured plants, industrial applications of micropropagation technique.

Suggested Readings

1. Razdan, M. K, An introduction to Plant Tissue Culture 2nd edn. Science Publishers, USA.

2. Bhojwani, S. S. and M. K. Razdan 1996.Plant Tissue Culture:Theory and Practice, Elsevier


3. Chrispeels, M. J. 2002. Plant Tissue Culture:Genetical Aspects. Jones and Bortlett

Publishers, International.

4. Chopra V. L. et al 1999. Applied Plant biotechnology. Science Publishers Inc.

5. Verpoorte, R. and A.W. Alfermann (Eds) 2000.Metabolic Engineering of plant secondary

metabolism, lower Academic Publisher